Doc 0000163369

- - .. . _·. ~ ::~ ·.:- ADDENDUM GROOMING ACTIVITY OF ALBINO EFF~CT-OF CHLORDIAZEPOXIDL AND INTRODUCTION Chlordiazepoxide and 1900 were employed to explore the usefulness of a test based on the grooming behavior of albino mice, according to the method of 0. Rohte, (Brit. J. Pharmac., ~:236, 1968) for the detection of sedative agents. Rohte states that the main advantage of this test is that it reduces the difficulties in testing weak sedatives by providing a releaser mechanism to the instinctive grooming movements of white mice. Rohte found that administration of certain test substances was associated with a significant inhibition of grooming movements compared with contro·l group. METHOD ~l-:~ Apparatus - Observation boxes were clear plastic with dimensions 3 ~:~t---~~ of 5. 5 x 10 x 5 in • The boxes ~Jere covered with wire screening .·_.\ .;· and the bottoms were lined with cardboard. Activated ca·rbon, Darco grade S-51 of the Atlas Powder Company, was used to cover the mice. All injections were made with a 25-gauge needle and .. glass syringes •. Subjects- The subjects were 60 female albino mice (19-31 grams), - HR/ICR strain supplied from the ··:~·;~:·~,.;:::-~~-":'~:;;..:. ·-.7~:;.:..:.; .. ;-;.:;~~~--:-:!-(~·f:-;:r • -- .. , ·- ~:.~:~::~~·~~ .r~ Procedure - The test mice were covered with pulverized charcoal and the intensity of blackening and its pattern were noted at 1.5 hour interv~ls up to 6 hours. The observations were graded = = on a 3-point scale: l white to light grey: 2 medium grey: = 3 dark grey to black. A. Chlordiazepoxide. Five subjects p~r dose level per day received· Chlordiazepoxide ·(Librium HCll intravenously ·l between 8 a.m. and 10 a.m. or a saline solution (control). Dose Iy, I...; i. ! levels of Chlordiazepoxide were 125 mg/kg, 63 mg/kg, 32 mg/kg, 3.2 mg/kg, and 0.32 mg/kg. Saline given at a level of 10 ml/kg (largest drug volume of solution injected). All animals at a single dose level were injected on the same day. After injection - . the animal was placed in a jar containing the powdered carbon and covered by shaking the jar. The subject was then removed by tail with forceps and placed· into a shoebox cage lined with saw- _,,. ,_.j dust for approximately 3 minutes. Subject w~s then placed into a plastic observation cage with ad libitum food and. water. Observations of the intensity and pattern of blackening were made a~ one The provided image is not a declassified CIA document but rather a promotional graphic for "The Black Vault," an online database of declassified government documents. The graphic features an illustration of a bank vault door on the left, with a glowing blue light emanating from behind it. On the right, white text, styled as "THE BLACK VAULT," is prominently displayed at the top. Below this title, explanatory text details the origin of the document from The Black Vault and specifically mentions the "MKULTRA/Mind Control Collection," noting that over 20,000 pages have been declassified by the CIA. The text concludes with a URL, "http://mkultra.theblackvault.com," presented in yellow. There are no photographs, handwritten annotations, official stamps, forms, diagrams, tables, redactions, or visual evidence of experimental procedures present in this graphic. The page is a typewritten document with handwritten annotations. There are several large, dark, textured areas that obscure portions of the page, suggesting redactions. These appear as large, irregular shapes, particularly in the lower half of the document. In the bottom right corner, there is a handwritten annotation in a circle: "175". The document contains no photographs, stamps, forms, diagrams, or tables. was then removed by tail with forceps and placed· into a shoebox cage lined with saw- _,,. ,_.j dust for approximately 3 minutes. Subject w~s then placed into a plastic observation cage with ad libitum food and. water. Observations of the intensity and pattern of blackening were made a~ one hour intervals according to the method of Rohte (1968) (see Figure 1,2). Observations were made on both dorsal (ventral } surfaces and each body area was noted as either dark, medium, or light (3,2, or 1, respectively). B. ~900. Two dose levels, 1 mg/kg and 0.1 mg/kg ®t.,.t ·?1 were tested in five mice each, according to the method used with Ill f2;":.;' Librium. ~t1 lt~;-1 i~ " ~ ;~tl~;~~Z&~~'?J~;i~t:~J~ITW~:1i'TZ:\:1CJ>S'!(:~JI@gf~~J,f~~~[i7f~~5!;;%~,;r;;;;;:;;·,;cq ,., .... ·· .. ~,~t'~t£¥s'"~ . '':~- -3- --· .. '~ :<~~ ~i ~1~1 RESULTS ~u The "grooming unit" appears to accurately measure grooming activity :" ,j during the first few hours of test. At later interva~s grooming becomes more difficult to measure be9ause the observer is unable to note additional grooming activity on areas which are already light. Therefore, the decreased rate of grooming toward the . .. : . . .sixth hour in ·all groups ex.cept the controls (see Figure 10) may- be due to the inability to measure grooming rather than an actual decrease caused by the drug. For purposes of statistical analysis, each subject was assigned a grooming index at each hourly observation (see Table 1). The grooming index was obtained by sunuiling the grey values (light = 1, medium= 2, dark = 3) for the six body areas a-f. Each dose group was compared with the controls in a Wilcoxon two-sample test (Laurence and Bacharach, p. 80) (see Table 2). The test was not applied to the one-hour data because of the large number of ties • ... l'· l·~ For Chlordiazepoxide, the grooming indices of the 125 and 63 mg/kg dose groups were found to be significantly decreased as compared : -~ to controls throughout the 6-hour period. The 32 mg/kg group :t showed a significant decrease in grooming at 2nd and 6th hour. ·j The grooming of the 3. 2 mg/kg group was found to be significantly :';,:~;;· ;~ ~~\:"~ decreased as compared to controls from the 3rd to the 6th hour. c;,,.,~.o··~ No difference in grooming was found with the 0. 32 mg/kg group. For~900, 0.1 mg/kg was a no effect level. The document page appears to be a typed research report, likely from a scientific experiment. It includes a page number "-2-" at the top. The text describes experimental procedures involving mice, charcoal, and substances like "Chlordiazepoxide (Librium HCl)". There are no photographs, handwritten annotations, official stamps, or filled-in forms visible on this page. However, there is dark, obscured content in the lower right corner of the page. Additionally, there are two instances where text has been redacted: one is labeled "B." followed by a redacted word and then "1900", and another appears to be a portion of text that was blacked out. ·j The grooming of the 3. 2 mg/kg group was found to be significantly :';,:~;;· ;~ ~~\:"~ decreased as compared to controls from the 3rd to the 6th hour. c;,,.,~.o··~ No difference in grooming was found with the 0. 32 mg/kg group. For~900, 0.1 mg/kg was a no effect level. Mice which received ~900 at 1.0 mg/kg showed a significant decrease (P = ~OS) at the 1-3 hour intervals and very significant decrease (P = 0.01) at the 4-6 hour intervals, when a one tailed test is considered • .. ·• " ·~::~,;~:?;a~:::~'-B~~0:J.!t~·'::iF_~;;~!ff£;::?:?·?~:JJ;~Jt:;;;:~§;.;!rw>~~.Gf'[t:r.~':;~~;J~~~7;~:~:)t?~;~:·r~~?S;;c~:r:::00:?rr:;-~---3~:s~::~r-~:LT~~ ·, ·.· .· .. . ' ·..: .. ,r ; .- . ·. . -. .. .. .· ... · "'! • .. ...L. . • !"'.: ·--==~ :··~~ ~ ':"f~' !" :_,.; . . . .. ,. .. ~~~ . .•.. _;._. .· .,,. . ·-· '· ·:~-;.,.·-. .: ·. - .. ·.~. . . ... ..:. ..~ _:............t. ., . . .. . . . . . .. ~- ,· .. ·-=-· .... "TI M€ (1-1·0i1f.~) ltrTI!:~ IAJ.l, TABLE 1. DISTRIBUTION OF VALUES OF THE GROOMING INDEX AT , I~~li EACH 1-HOUR OBSERVATION FOR 5 DOSE LEVELS OF- .. LIBRIUM HCl AND FOR SALINE CONTROLS Librium HCl (mg/kg) Saline (10 ml/kg) Hour 125 63 . 32 . 3.2 0 :·32 1 18 17 17 17 17 17 15 17 17 ~7 18 17 ~7 17 17 17 17 17 15 17 17 17 17 17 17 1a 11 16 15 17 18 18 17 16 17 17 17 17 17 17 18 17 17 17 15 18 17 18 17 1'1 2 18 16 17 17 17 15 12 16 15 17 18 17 16 17 16 14 17 16 14 17 17 17 17 16 14 17 12 15 13 16 18 17 17 16 13 14 16 15 14 14 18 17 16 15 12 16 12 18 13 16 3 18 16 17 17 17 i2 8 13 15 17 ~(:.0:~ ;f!;, 18 17 14 17 15 13 12 15 12 14 17 17 16 16 13 17 ·9 15 12 14 18 16 15 15 13 11 15 14 13 14 18 17 11 14 12 14 9 17 12 15 4 18 16 17 17 16 1i 8 12 12 16 18 17 13 17 13 13 10 15 12 13 17 17 14 14 13 16 7 12 11 The document page consists primarily of typed text, with a page number "-3-" at the top and the heading "RESULTS". There are no photographs, handwritten annotations, stamps, forms, diagrams, or tables visible on the page. One area of the text, within the last paragraph of the "RESULTS" section, appears to be partially redacted or obscured, making it difficult to read the specific drug or substance being discussed. The visual content of this page is dominated by dense blocks of printed words, indicating it's a report or research paper. 18 16 15 15 13 11 15 14 13 14 18 17 11 14 12 14 9 17 12 15 4 18 16 17 17 16 1i 8 12 12 16 18 17 13 17 13 13 10 15 12 13 17 17 14 14 13 16 7 12 11 13 ·18 16 13 14 11 9 13 12 13 14 18 17 11 13 10 12 11 17 12. 14 5 18• 16 17 17 13 10 8 12 11 14 18 17 11 17 13 7 10 12 8 13 17 17 13 14 11 15 7 10 11 13 18 16 13 13 10 8 9- 11 13 14 18 17 11 11 9 10 7 17 11 14 6 18 16 17 17 13 8 8 10 8 14 17 17 11 16 11 7 9 11 6 11 17 17 13 13 11 11 7 8 8 13 17 16 13 11 10 7 7 10 10 11 17 17 11 11 9 9 7 15 7 11 n 5 5 5 5 5 25 ;,3m .. :.':::,f;,ll ·. . :~~:i . , ~.[~:o:tl ..... " -~i -~+t:~J • TABLE 2. -VALUES FOR A TWO•TAILED TEST ON 5 DOSE LEVELS OF LIBRIUM HCl .. Wllcoxon 2-Samnle Test*- Librium HCI . '· Hour 125 mg/kg 63 rng/kg 32 mg/kg 3.2 rng/kg 0.32 mg/kg 2 <. 0. 01 0.02 0.05 ( )0 :o5) { ~.OS) <o 3 .01 ( >O.OS) 0.05 ( )0. OS) 4 ,0.01 /"O.OS) 0.02 ( )0. OS) 5 ,0.01 70. OS) o-. 02 ..co. 6 01 0.01 .(o. 01 ( ( ) = Not significant * Rurnke, C. L. , and de Jonge H. Chapt. 3, Design, Statistic.al 1 1 and Interpretation in Vol. I Evaluation of Drug Analys~s Activities: Pharrnacornetrics ed. by Lawrence, D. R., and Bacharach, A. L., Academic Press, N.Y. 1964 .. ·• TJ1.BLE 3. DISTRIBUTION OF VALUES OF GROOMING INDEX AT 1-HOUR OBSERVA.. TION FOR EACH OF 2-DOSE LEVELS OF 900 Scores for Individual Mice _.._1900 1 rng/kg 11''1~00 -· 0.1 rng/kg -Hour _!. ....1.. 2 ...!. .2. ..i. ~ J..Q_ 1 18 18 18 18· 18 17 15 17 18 16 2 17 17 18 17 17 15 11 17 .14 10 3 16 17 17 17 16 12 12 17 12 8 4 16 16 17 16 16 12 11 13 11 7 5 16 14 . ~ J..Q_ 1 18 18 18 18· 18 17 15 17 18 16 2 17 17 18 17 17 15 11 17 .14 10 3 16 17 17 17 16 12 12 17 12 8 4 16 16 17 16 16 12 11 13 11 7 5 16 14 . 17 15 12 11 10 10 10 6 6 14 14 16 15 12 10 7 10 8 6 -. ·.. . ..;, . .- '~ t;~ ~ :,~·~ --~ TABLE 4. :i~:\~.:Ji!:·l.-~~:..~-~.·x .. ... J'7~·~."5o- 1\ " ::. ' : '- VALUES FOR 2-TAILED TEST 0 0.1 MG -'··: ........ i. 1 ~·''I VERSUS .. .-.1900 1.0 MG/KG DOSE LEVELS i ~~:i ~! Hour p Values ...... •• h ~~~ ., :'~ 1 .OS 2 .OS 3 .10 4 .02 ... ._.:,-, . : .. • .. i ~ ~f~ .·.~·· :~~ 5 .02 X~ :,:'V ~-::.;·;~ ~i: :-:{~~ ::;)·t ':..::~ ~ 6 .02 ~~~j' •·r"" 'if ~~ ;;~~~~ ilg -~'~·;, II <t! .~ /:~\ ~'f. !~.! ·. ltr":t~:!ft;'i);J{~~-,,;;;;'i:J;~~~!;:'0J•''<?,.=•i"'"·f~_'.:TSW7""~;;:"'~'{'~~f"2)3,'2'""'!C","'<''""fC~~::v;r~ ;'e {~f~'mit;t.§;{~ci::;;:_e ~·• ~-;"'~"··· "'" .L:;;_)JzL j_:··svx.jj;c,,,);· >.]. c:sic.~''·· .. ..:.:.. ;~;~· ~·-··· : -~;!:~~ LDSO 36.0 -28~0-45oO­ MED50 Oo56 -0.18-loS-· RATIO LD50/MEDSO 63.0 STATE SOLID tF~-~ INTRAVENOUS TOXICITY TO MICE DOSE DILUT REACT. REACTION SIGN MINQ "DEG- MIN. TO MG/KG ML/KG 'FRACTo APPR REE RECOVER 100.0 10.0 212 ·DEATH 74 0 -32.0 3.2 '212 DEC LOCOMOTOR ACTIVITY 124 30 * 120 32.0 3.2 2/2 DEC SENSITIVITY TO PAIN 229 30 180 32.0 3•2 2/2 INC SENSITIVITY TO SOUND 130 0 "*** 300 32.0 -3.2 '2/2 INC SENSITIVITY TO TOUCH 131 0 G 300 1 ~-t. -32.0 -3.2 2/2 DEC REARING 'FREQUENCY 632 30 120 ~~ .l! 32~0 3o2 212 -STRAUB TAIL 233 0 15 32o0 -3.2 '2/2 MIXED ·coNVULSIONS 336 0 15 32.0 -3.2 '212 AUDIOGENIC SEIZURE 536 0 30 -32.0 -3.2 •2/2 -DEC PREENING 240 8 :.zo 32o0 3.2 '2/2 ·RUBS I NG NOSE 340 8 30 32.0 3.2 212 TREMORS-REST AND MOVEMENT 144 0 30 32.0 3.2 2/2 BLINKING=EXCESSIVE 247 8 30 32.0 3.2 ~/2 'EYELID PTOSIS-NONPARALYTIC 149 8 * ~20 32o0 3.2 '2/2 PUPILLARY LIGHT REFLEX ABS 152 0 240 32.0 3.2 2/2 MYDRIASIS 154 0 ** 1440 -32.0 3.2 212 SALIVATION 57 8 *** 60 32.0 3.2 2/2 INC URINATION 158 30 60 32.0 3.2 l/2 INC RESPIRATORY DEPTH 161 0 a 32.0 3.2 2/2 IRREGULAR RESPIRATORY DEPTH361 0 60 ll -. '""''~ '~ ,; This declassified document page features a handwritten graph with axes labeled "AVERAGE NUMBER OF BODY AREAS PORS" and "TIME (HOURS) AFTER INJ.". The graph displays bar-like data points representing measurements for "LIGHT", "MEDIUM", and "DARK" categories. Handwritten text above the graph indicates "Fig. 1", "Saline (10 ml/kg) - Change in the average number of dark, medium, & light areas over a six hour period after injection; n = 25". There is a black ink smudge obscuring a portion of the graph's horizontal axis. There are no photographs, stamps, or other forms of official markings present. The document appears to be a graph with handwritten annotations. The graph has a Y-axis labeled "AVERAGE NUMBER OF BODY AREAS PERS" and an X-axis labeled "TIME (HOURS) AFTER INJ.". There are bars plotted on the graph, representing data points with labels "LIGHT", "MEDIUM", and "DARK" near the top. A title above the graph reads "Librium HCl (0.32 mg/kg i.v.) Change in the average number of dark, medium, & light areas over a six hour period after injection. n=5". There are also handwritten notations and what appear to be redactions or obscured text in the upper right and lower center of the page. The overall document is on lined graph paper. ABS 152 0 240 32.0 3.2 2/2 MYDRIASIS 154 0 ** 1440 -32.0 3.2 212 SALIVATION 57 8 *** 60 32.0 3.2 2/2 INC URINATION 158 30 60 32.0 3.2 l/2 INC RESPIRATORY DEPTH 161 0 a 32.0 3.2 2/2 IRREGULAR RESPIRATORY DEPTH361 0 60 ll -. '""''~ '~ ,; ..- ,. ·\"'f ~~~~2J -~~~t~;z;?:if~~ti'!B\ii~l¥:Z;t~~;:r0'~i~~;10·~·*·1~~F'~iW'f[~h!t~%~0~~~~::n:r;\t~D;· MIN. DEG M!N. TO --- "lJ APPR REE RECOVER -;~ 32.0 3.2 l/2 INC RESPIRATORY RATE 162 0 8 II -.. * 32.0 3.2 '2/2 IRREGULAR RESPIRATORY RATE 362 0 60 32o0- 3o2 '2/2 MOT DEF HORIZONTAL WIRE 171 0 *** 60 32o0 -3.2 2/2 ~OT DEF VERTICAL SCREEN 271 0 *** 60 32.0 3.2 2/2 MOT DEF HORIZONTAL STRIP 471 0 *** 60 32o0 3.2 2/2 M~T DEF VERTICAL ROD 371 0 *** 60 32.0 ·-3.2 2/2 MOT DEF ROTA•ROD 771 0 *** 60 32.0 3.2 2/2 MOT DEF INCLINED STRIP 671 0 *** 60 32o0 3._2 1/2 ABNORMAL VIBRISSAE l72 8 60 32.0 -3.2 2/2 LOW :CARRIAGE . 175 8 60 . * 10.0 10.0 l/2 DEC LOCOMOTOR ACTIVITY 124 30 120 10o0 10.0 2/2 DEC ·SENSITIVITY TO PAIN 229 ·30 180 10a0 10.0 '2/2 INC SENSITIVITY TO TOUCH 131 0 G 300 10.0 10.-0 1/2 ·DEC REARING FREQUENCY 632 30 120 10o0 10.0 1/2 MYDRIASI-S 154 30 ** 120 10.0 10.0 2!2 -SALIVATION 57 8 *** 60 ~~~--~ 10.0 10.0 2/2 IRREGULAR RESPIRATORY DEPTH36l 0 30 I to.o ·10.0 2/2 tRREGULAR RESPIRATORY RATE 362 0 30 ~d -3.-2 1/2 DEC SENSITIVITY TO PAIN 229 15 . .laO 3.2 '2/2 INC SENSITIVITY TO TOUCH 131 0 * 300 G 1.0 10.0 l/2 ABNORMAL REACTION TO PAIN -429 120 G 240 leO 10.0 2/2 INC SENSITIVITY TO TOUCH 131 15 G 240 - 2/2 NO EFFECT 73 J ·;r,..,_ ·5.0 '2/2 DEATH 74 0 ~~:r-~=~ 50.0 ;t ~~~ 40.0 ·212· DEATH 74 0 ~W-::~"h.c~~]1 32.0 0 '2 DEATH 74 0 25.0 0/2 DEATH 74 0 OIL. 'H20 QS C H20 -~:-..: ~ ~~ :~1~~ih~£.;:;:,g,, L ~ --·---·_ ,;· .:: • ..i:L:C_;,._c -• c :; :.c·• >;.:;c;.;_.L;; 2367 w l ::;,r:~~ d .::·~~ ifl LD50 too.o .·-63o0-160·0- MED50 1.8 -0.56-5.6-· RATIO LD50/MED50 56.0 -STATE SOLID ~;"'~. ~ ~1 ~ ~c~~~ ~~~g: !NTRA~~~g~io~o;;~~TY DOSE TO MICE MINo "OEG- MIN. TO APPR REE RECOVER ~~ tiijG 1.0 l:/2 DEATH 74 0 1.0 l/2 DEC.LOCOMOTOR ACTIVITY 124· 0 180 1.0 1/2 DEC SENSITIVITY TO TOUCH 231 0 60 This document displays a bar graph on graph paper, likely representing experimental data. The title "Librium HCl (3.2 mg/kg 1.1.)" and subtitles "LIGHT," "MEDIUM," and "DARK" suggest a study involving the drug Librium. The X-axis is labeled "TIME (HOURS) AFTER INJ." and the Y-axis "AVERAGE NUMBER OF BODY AREAS PER S." Handwritten annotations and a scale of 1 to 6 on the Y-axis are visible, along with the approximate sample size "n=5." There is no photographic content, but the graph's structure implies controlled observation and data collection. too.o .·-63o0-160·0- MED50 1.8 -0.56-5.6-· RATIO LD50/MED50 56.0 -STATE SOLID ~;"'~. ~ ~1 ~ ~c~~~ ~~~g: !NTRA~~~g~io~o;;~~TY DOSE TO MICE MINo "OEG- MIN. TO APPR REE RECOVER ~~ tiijG 1.0 l:/2 DEATH 74 0 1.0 l/2 DEC.LOCOMOTOR ACTIVITY 124· 0 180 1.0 1/2 DEC SENSITIVITY TO TOUCH 231 0 60 m:g .1.0 l/2 DEC SENSITIVITY ·To SOUND 230 0 60 !~ 1.0 1/2 DEC PREENING 240 -0 ,120 1.0 l/2 :EXTENSION OF LIMBS 341 0 . 15 'l igg:g ~ft: 1.-0 l/2 'PROSTRATION -43 0 15 1.0 1/2 EYELID PTOSIS-NONPARALYTIC 149 8 "*** 180 ~i] 1oo.o 1.o 1/2' PUPILLARY .LIGHT REFLEX ABS 152 15 60 ~~~~.';. :_-~_:' 1 1 0 o 0 o • . o 0 1.0 1/2 MYDRIASIS 154 15 60 -"Bl:, i 1.0 l/2 DEC RESPIRATORY DEPTH 261 0 30 -:~~~}. ~-~ 100.0 1.0 1/2 INC RESPIRATORY DEPTH 161 -30 120 too.o 1.0 1/2 "IRREGULAR RESPIRATORy DEPTH361 0 30 100.0 1.0 1/2 DEC RESPIRATORY RATE 262 30 120 too.o 1.o 1/2 IRREGULAR RESPIRATORY RATE 362 0 30 100.0 1.0 1/2 MOT DEF HORIZONTAL WIRE 171 0 *** 15 too.-o 1.0 1/2 MOT DEF VERTICAL SCREEN 271 0 *** 15 1oo.o .1.o 1/2 MOT DEF HORIZONTAL STRIP 471 0 •*** 15 i : 1.0 1/2 MOT ·DEF VERTICAL ROD 371 0 30 .;-\·~' -~ ~ ~ ~ ·1.0 1/2 MOT DEF ROTA-ROD 771 0 15 ~~100,0 1.0 1/2 MOT DEF INCLINED STRIP 671 0 ***' 30 ~n "! ,,.,> .. ·- ,ff~~~%;:¥,':ff:i)J!Jz;L •.. • • . . .. .. . . ·''"'"'~.sc;~~2b80L::. t;£1:;; :~ fX':'•:•,':;_;,••15:ZL ,::.., ~;_._·z;-;,·, w· . " ;.S ,).' :.: ·:..-~ -~~r.:.:·:;; . INTRAVENOUS TOXICITY TO M!CE ?~~~ DOSE DILUT RFACTo REACTION SIGN MINo DEG MIN. TO MG/KG ML/KG FRACTo APPR REE RECOVER ~i 32.0 0.64 2/2 DEC LOCOMOTOR ACTIVITY 124 0 *** 120 32.0. 0.64 l/2 DEC SENSITIVITY TO PAIN 229 30 - .. * 60 32.-0 0.64 1/2 ~. A6NORMAL REACTION TO PAIN 429 30 60 32.0 Oo64 2/2 DEC SENSITIVITY TO TOUCH 23~ 0 * 60 32.0 ,· 0.64 2/2 SOCIAL INTERACTION ALTERED 132 0 180 32.0 0.64 2/2 DEC REARING FREQUENCY 632 0 120 32.0 0.64 2/2 ATAXIA 35 0 ·*** 15 32o0 0.64 2/2 DEC PREENING 240 0 180 32.0· 0.64 1/2 LOW POSTURE 241 0 30 32.0 0.64 2/2 EXTENSION OF LIMBS 341 0 30 32.0 Oo64 2/2 EYELID PTOSIS-NONPARALYTIC 149 8 *** 120 32.0 0.64 1/2 PUPILLARY LIGHT 632 0 120 32.0 0.64 2/2 ATAXIA 35 0 ·*** 15 32o0 0.64 2/2 DEC PREENING 240 0 180 32.0· 0.64 1/2 LOW POSTURE 241 0 30 32.0 0.64 2/2 EXTENSION OF LIMBS 341 0 30 32.0 Oo64 2/2 EYELID PTOSIS-NONPARALYTIC 149 8 *** 120 32.0 0.64 1/2 PUPILLARY LIGHT REFLEX ABS 152 8 60 32.0 0.64 1/2 MYDRIASIS 154 8 ** 60 -32.0 0.64 2/2 INC RESPIRATORY DEPTH 161 0 120 32.0 Oo64 2/2 IRREGULAR RESPIRATORY DEPTH361 0 30 32.0 0.64 2/2 DEC RESPIRATORY RATE 262 0 120 32.0 0.64 2/2 IRREGULAR RESPIRATORY RATE 362 0 30 32.0 0.64 1/2 MOT DEF HORIZONTAL WIRE 171 0 *** 15 32.0 0.64 1/2 MOT DEF VERTICAL SCREEN 271 0 *** 15 32.0 0.64 2/2 MOT DEF HORIZONTAL STRIP 471 0 ***' 30 o· -32.0 0.64 1/2 MOT DEF VERTICAL .ROD 371 '*** 15 32.0 0.64 1/2 MOT .DEF ROTA-ROD 771 0 *** 30 tH 32.0 0.64 1/2 MOT DEF INCLINED STRIP 671 0 ***' 30 10.0 1.0 2/2 DEC .LOCOMOTOR ACTIVITY 124 0 '*** 120 .. 10.0 .1.0 1/2 DEC SENSITIVITY TO PAIN .229 30 !.80 ~;:~-~- ~~ 10.0 1.0 2/2 DEC -SENSITIVITY 'TO TOUCH 231 0 *- 60 10.0 1.0 2/2 SOCIAL INTERACTION ALTERED 132 0 180 10.0 1.0 2/2 DEC REARING FREQUENCY ,632 0 120 10.0 1.0 ·DEC PREENING 240 ·0 120 '2~2 10.0 1.0 1/2 .LOW POSTURE 241 30 180 10.0 1.0 2/2 EYELID PTOSIS-NONPARALYTIC 149 8 '*** 120 10.0 1.0 2/2 IRREGULAR .RESPIRATORY DEPTH361 0 30 10.0 1.0 2/2 IRREGULAR RESPIRATORY RATE 362 0 30 10.0 1.0 1/2 MOT DEF HORIZONTAL WIRE 171 0 *** 8 .10.0 1.0 1/2 MOT DEF VERTICAL SCREEN 271 0 ***·· 8 .10.0 1.0 1/2 MOT DEF HORIZONTAL STRIP 471 0 *** 30 10o0 1.0 1/2 MOT DEF VERTICAL ROD 371 0 *** 8 10o0 1.0 1/2 MOT DEF ROTAoROD 771 0 *** 15 10.0 1 0 1/2 MOT DEF INCLINED STRIP 671 0 *** 30 0 ·'~] 2/2 DEC LOCOMOTOR ACTIVITY 124 30 * 120 2/2 SOCIAL INTERACTION ALTERED 132 30 120 2/2 DEC REARING FREQUENCY 632 30 1.20 ·i ;>,'-.• .. . INTRAVENOUS TOXIC!TY TO MI·CE DOSE DILUT REACT. REACTION SIGN MINo DEG MtN. TO APPR REE RECOVER MG/KG ML/KG FRACTe 2/2 DEC PREENING 240 30 120 2/2 'EYELID PTOSIS-NONPARALYTIC 149 30 * 120 1.0 o.so 2/2 NEl EFFECT 73 130.0 '2/2 DEATH 74 0 0.79 0/2 DEATH 74 0 The image contains a single page of a typed document with a table presenting data. The table, titled "TABLE 1. DISTRIBUTION OF VALUES OF THE GROOMING INDEX AT EACH 1-HOUR OBSERVATION FOR 5 DOSE LEVELS OF LIBRIUM HCL AND FOR SALINE CONTROLS," is the primary visual element. It is divided into columns representing different dosages of Librium HCl and Saline, with rows indicating the hour of observation. There are no images of people, locations, equipment, or subjects. No handwritten annotations, signatures, or official stamps are visible. The document appears to be a declassified MKUltra file, but there are no visual cues on this page to suggest experimental procedures or facilities. The bottom of the page shows a blacked-out area, likely a redaction. The document is a page from a report containing a table of data. The table, titled "TABLE 2.", presents numerical values under the heading "Wilcoxon 2-Sample Test* Librium HCI", with columns representing different dose levels and rows representing hours. Handwritten annotations are absent, and there are no official stamps, photographs, or diagrams. There are no filled-in fields or visual evidence of experimental procedures. However, there is a note at the bottom, "( ) = Not significant", and a footnote referencing a publication. There are also some small, dark redactions or scribbles toward the bottom right corner of the page. .. . INTRAVENOUS TOXIC!TY TO MI·CE DOSE DILUT REACT. REACTION SIGN MINo DEG MtN. TO APPR REE RECOVER MG/KG ML/KG FRACTe 2/2 DEC PREENING 240 30 120 2/2 'EYELID PTOSIS-NONPARALYTIC 149 30 * 120 1.0 o.so 2/2 NEl EFFECT 73 130.0 '2/2 DEATH 74 0 0.79 0/2 DEATH 74 0 0.63 1/2 DEATH 74 0 100% PEG 300 QS C 100% PEG 300 ·DlLe iH II . •l ·,~ ll~ ..; .:·.:r ~ ,<-=~··'If ~f. III 'i ~~- -- .• ·. ~:t~~:~l ~,~~<;;~:':l!~;~)~fh'•-·?',ff;'il!~'i";D:~S~S~J~;\};~MtW::;~:'Z~';';'~1;:;:;;JttJ;;'1f'i'";h-·~?5'&2~:- -79.o-tzo.o- -t.a-tsoo- 18.0 INTRAVENOUS TOXICITY "TO MICE REACTION SIGN MINo DEG MIN. TO APPR REE RECOvER w 2516 2 INTRAVENOUS TOXICITY TO MICE DOSE DILUT REACT. REACTION SIGN MINo DEG MINo TO MG/KG ML/KG FRACTo APPR REE RECOVER 100.0 10.0 1/2 PILOERECTION 72 60 240 100.0. 10.0 1/2 LOW CARRIAGE 175 0 240 ~ 32.0 3.2 2}2 DEC LOCOMOTOR ACTIVITY 124 15 * 60 32.o· , 3.2 2/2 QEC REARING FREQUENCY 632 15 60 32.0 3.2 l/2 RUBBING NOSE 340 8 30 32.0 3.2 '2/2 ·:UNUSUAL POSTURE 541 0 15 32o0 3.2 2/2 EYELID PTOSIS-NONPARALYTIC 149 15 *** 120 32.0. 3.2 '2/2 PUPILLARY LIGHT REFLEX ASS 152 8 180 32.0 3.2 . MYDRIASIS 154 8 ** 180 -~12 .32.0 3.2 2/2 INC RESPIRATORY DEPTH 161 0 30 ·o 32.0 3.2 INC-RESPIRATORY RATE 162 30 ~2/2 32.0 3.2 1/2 ~OT DEF ROTA-ROO -771 8 30 32.0 -3.2 '2/2 LOW CARRIAGE 175 0 30 • 10.0 10.0 ·212 EYELID PTOSIS-NONPARALYTIC 149 30 •*** 120 10.0 10~0 2/2 PUPILLARY .LIGHT .REFLEX ASS 152 8 120 10.0 10.0 2/2 MYDRIASIS 154 8 120 : 10.0 10.0 ·212 IRREGULAR RESPIRATORY OEPTH361 0 30 ~10.0 10.0 l/2 IRREGULAR RESPIRATORY RATE 362 0 30 .. -3.2 '2/2 NO EFFECT 73 ·120.0 12.0 2/2 ·DEATH 0 .ao .o 8.o 0/2 DEATH 74 0 l~J 0/2 DEATH 74 0 63.0 ~¥Ji ~ 100% STEROL DILo SUSP QS C H20 'DILo · ... ~,. ~ ,--~-:-~ ~) II -~~~~~ .?1 .. .LD50 110.0 ·-89o0-l40.0- MED50 3. 2 -1. o-ro. o-· RATIO LD50/MED50 35.0 ,STATE SOLID INTRAVENOUS TOXICITY 'TO MICE DOSE 'D I LUT REACT • REACTION SIGN MINo '·DEG-· MIN. TO MG/KG ML/KG FRACTo APPR REE RECOVER roo.o 1o.o 2/2 ·DEC .LOCOMOTOR ACTIVITY 124 0 "*** 300 roo.o 10.0 '2/2 DEC ·SENSITIVITY 'TO 'TOUCH .231 0 60 100.0 10.0 '2/2 ·SOCIAL INTERACTION ALTERED .132 0 60 100.0 10,.0 212 DEC REARING FREQUENCY 632 0 300 roo.o 1o.o· The image displays a table titled "TABLE 3. DISTRIBUTION OF VALUES OF GROOMING INDEX AT EACH 1-HOUR OBSERVATION FOR EACH OF 2 DOSE LEVELS." The table presents numerical data, likely results from a scientific experiment, organized by "Hour" and showing "Scores for Individual Mice" under two different "Dose Levels" (-1900 1 mg/kg and -1900 0.1 mg/kg). There are no photographs, handwritten annotations, or official stamps visible. A portion of the document has been redacted, indicated by a black marker obscuring text at the top of the page, and a separate, smaller redacted area appears towards the bottom right. The visual content primarily consists of structured data presented in a tabular format. MINo '·DEG-· MIN. TO MG/KG ML/KG FRACTo APPR REE RECOVER roo.o 1o.o 2/2 ·DEC .LOCOMOTOR ACTIVITY 124 0 "*** 300 roo.o 10.0 '2/2 DEC ·SENSITIVITY 'TO 'TOUCH .231 0 60 100.0 10.0 '2/2 ·SOCIAL INTERACTION ALTERED .132 0 60 100.0 10,.0 212 DEC REARING FREQUENCY 632 0 300 roo.o 1o.o· 1/2 .EXTENSION OF LIMBS 341 0 30 100.0 10.0 l/2 RIGHTING REFLEX DEPR 742 ·O ·8 100.0 10.0 ··2/2 =PROSTRATION ··43 0 30 100.0 10.0 '2/2 'EYELID PTOSIS-NONPARALYTlC 1·49 .15 *** G 300 100.0 10.0 212 INC RESPIRATORY DEPTH 161 0 180 100.0 10.0 '2/2 DEC RESPIRATOR~ RATE 262 0 180 100.0 10.0 '2/2 MOT ·DEF HORIZONTAL WIRE 171 0 *** 60 100o0 1o.o '2/2 MOT DEF 'VERTICAL SCREEN 271 0 *** 60 too.o 10.0 212 MOT DEF HORIZONTAL STRIP ·471 0 ***- 120 100.0 10.0 2/2 MOT DEF VERTICAL ROD 371 0 *** 60 100.0 10.0 . 2/2 MOT DEF ROTA-ROD ·771 0 60 1oo.o 10.0 2/2 MOT DEF INCLINED STRIP 671 0 120 too.o 10.0 212 LOW :CARRIAGE 175 30 60 ·32.0 '212 DEC .LOCOMOTOR ACTIVITY 124 e ***' 120 32.0 '2/2 SOCIAL INTERACTION ALTERED 132 0 60 32.0 '212 DEC REARING FREQUENCY ' 632 0 60 ·. ~~r\%~~S:~L:: ~EAC~.~=:NTRA~~~g~~ 0~0~ i~~ TY TO MICE MINo DEG-· M( N. TO ·.,::;-~ {lj MG/KG ML/KG FRACT • APPR REE RECOVER 32.0 ·3·2 '2/.2 'EYELID PTOSIS-NON~ARALYTIC 149 15 60 32.-0 .. 3,2 '2/2 INC RESPIRATORY DEPTH 161 0 60 32.0 3.2 212 .. DEC RESPIRATORY RATE 262 0 60 32.0 3.2 '2/2 MdT DEF HORIZONTAL STRIP 471 0 30 32.0 ·3.2 l/2 MOT DEF VERTICAL ROD 371 0 30 32.0 ··3•2 '2/2 MOT DEF INCLINED STRIP 671 0 *** 30' lOeO '2/2 'EYELID PTOStS-NONPARALYTIC 149 60 240 EYELID PTOSIS-NONPARALYTIC 149 60 240 ~/2 1/2 NO EFFECT 73 leO 10.0 '2/2 'NO EFFECT 73 160.0 '2/2 DEATH 74· 0 .120.0 12.0 '2/2 DEATH 74 0 0/2 DEATH 74· 0 DlL. 100% ·STEROL ·DIL. SUSP QS .C H20 ! fi:;)' ~'~·}l ~;,;~ :~ ~v-~l :l ~1.'~ !.,_'(..~-('(' .-·~f: If:) ~..;,vI II ~~~: -~ .. ·. ,._e _. _- .:/j~~~;;n:}:{t3Ds;;t-c::I _. :::~~-~,~;..:.~;:i.~::.;;;.;.:~i:~.x;,;-·;··:. . ..,.;:.:~if~;:J(ii~~---~~<;;:·~ .. , :::·-~..:.c.:_,,-. : .. _- .. ·· .. -·· ·.·-' ' :~~:f~~;l PAGE 2598 INTRAVENOUS TOXICITY TO MICE DOSE DILUT REACTo REACT I ON ·sIGN MINo' DEG MIN. TO ( 11G/KG ML/KG FRACTo AP"P'R REE RECOVER .. o.032 3.2 '2 ~ / 2. HEAD TWITCH 531 120 * ..,.;:.:~if~;:J(ii~~---~~<;;:·~ .. , :::·-~..:.c.:_,,-. : .. _- .. ·· .. -·· ·.·-' ' :~~:f~~;l PAGE 2598 INTRAVENOUS TOXICITY TO MICE DOSE DILUT REACTo REACT I ON ·sIGN MINo' DEG MIN. TO ( 11G/KG ML/KG FRACTo AP"P'R REE RECOVER .. o.032 3.2 '2 ~ / 2. HEAD TWITCH 531 120 * 240 o.032 3.2 1/2_ INC PREENING 140 120 G 300 Oo032 3.2 1/2 INC SCRATCHING 440 180 G 300 o.032 3.2 2/2 EYELID PTOSIS-NONPARALYTIC 149 120 *** G 300 o.032 3.2 2/2 PHOTOPHOBIA ·53 120 *** G 300 Oo032 3•2 2/2 PILOERECTION 72 120 G 300 Oo032 3.2 1/2 SKIN FLICK 79 120 240 0.010 10.0 2/2 DEC LOCOMOTOR ACTIVITY 124 15 ·*** G 120 0.010 10.0 21? HEAD TWITCH . 531 15 * G 120 OoOlO 10.0 2/2 INC PREENING 140 15 G 120 OoOlO 10.0 1/2 RUBBING NOSE 340 30 G 120 0.010 10.0 1/2 INC SCRATCHING 440 .15 G 120 0.010 10.0 1/2 PILOERECTION '72 15 G 120 0.010 10.0 2/2 SKIN FLICK 79 15 G 120 3o2-3 2/2 DEC LOCOMOTOR ACTIVITY 124 30 *** G 120 3.2-3 2/2 HEAD TWITCH 531 30 * G 120 3.2-3 2/2 -INC PREENING 140 8 G 120 ~7>~ ~~ 3.2-3 '2/2 SKIN FLICK 79 15 G 120 ~H 1.0-3· 10.0 1/2 LICKING ·coMPARTMENT WALLS 825 30 G 120 1.0-3 10.0 1/2 INC PREENING 140 60 G !.20 ~~- ;~~ ( 1.0-3 10.-o 1/2 NO EFFECT 73 3.2-4 1/2 .LICKING COMPARTMENT WALLS 825 30 G 120 ~-; ·a 3.2-4 1/2 INC PREENING 140 30 G 120 ~,:~ 3.2-4 1/2 NO EFFECT 73 ~:1-~ ·a.o '2/2 · · :DEATH 74 0 25.0 ·5.o 0/2 DEATH 74 0 ·2o.o 0/2 ·DEATH 74 ·0· DILo 'Ool .N HCL QS ·c H20 ~·~hi ·._-;0;~'"E-. ·I~ .. ·. ~i~;;0,t~~;j)t':Z"'¥!J0'ii':l'7~\~~2C,yt(i'2'\~"'r~;;;f~}{'fif1~'ci;~ ;T~''00';s;'k;r~0~:SY:fiii;;jJC[f'f,:f~;G[;?)7' PAGE 2598 INTRAVENOUS TOXICITY 'TO MICE DOSE DILUT REACT. REACTION. SIGN MIN. DEG• MiN. TO ( }\G/KG ML/KG 'FRACTe APPR REE RECOVER .. . 3.2 3,2 Z/2.· DEC LOCOMOTOR ACTIVITY 124 120 * G 300 .3.2 -3,2 1/~ LICKING ·cOMPARTMENT WALLS 825 60 120 3o2 -3.2 2/2 INC SENSITIVITY 'TO PAIN 129 60 300 3•2 3,2 '?/2 INC SENSITIVITY TO TOUCH 131 8 *** G 300 3,2 3,2 '2/2 HEAD TWITCH 531 30 300 3.2 -3.2 2/2 SOCIAL INTERACTION ALTERED 132 30 120 3.2 3.2 ·z12 DEC REARING FREQUENCY 632 30 180 3,2 3,2 l/2 DEC PREENING 240 30 60 3.2· 3,2 t/2 INC PREENING 140 The image displays a typed document featuring a table with experimental data. The table is titled "TABLE 4" and presents "P VALUES FOR 2-TAILED TEST OF 1900 0.1 MG/KG VERSUS 1900 1.0 MG/KG DOSE LEVELS." It lists hours from 1 to 6 alongside corresponding "P Values." There are some black ink smudges or marks at the top and bottom of the page, and one heavily redacted section obscures some text within the table's caption. The overall document appears to be a scientific or statistical report. '?/2 INC SENSITIVITY TO TOUCH 131 8 *** G 300 3,2 3,2 '2/2 HEAD TWITCH 531 30 300 3.2 -3.2 2/2 SOCIAL INTERACTION ALTERED 132 30 120 3.2 3.2 ·z12 DEC REARING FREQUENCY 632 30 180 3,2 3,2 l/2 DEC PREENING 240 30 60 3.2· 3,2 t/2 INC PREENING 140 120 G 300 3,2 3,2 1/2 RUBBING NOSE 340 8 30 3o2 -3.2 '2/2 INC SCRATCHING -440 180 300 -3.2 3.2 1/2 EYELID PTOSIS-NONPARALYTlC 149 60 300 3.2 3,2 1/2 PHOTOPHOBIA 53 60 300 ·3e2 3.2 '2/2 PILOERECTION 72 60 300 3.2 3.2 '2/2 SKIN FLICK 79 120 G· 300 ::a ~~:~)- 1.0 10,0 1/2 DEC .LOCOMOTOR ACTIVITY 124- 30 *** .G 300 1,0 10,0 '1/2 .LICKING :COMPARTMENT WALLS 825 120 180 1.0 10,0 '2/2 INC SENSITIVITY 'TO TOUCH 131 8 300 1.0 10,0 t/2